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K. Vijayabhaskar ¹, Macharla Sravanprasad ², Goli. Venkateshwarlu ¹, P. Suvarna Devi ³, K. Hemanth Kumar ⁴, J. Sunil ⁵

Affiliations
1 Venkateshwara Institute of Pharmaceutical Sciences, Nalgonda, Andhra Pradesh-508001, IN
2 GBN Institute of Pharmacy, Ghatkeshar, RR District, Andhra Pradesh-501301, IN
3 Saipranavi College of Pharmacy, Keesara, RR district Andhra Pradesh-501301, IN
4 Shanta College of Pharmacy, Huzurabad, Karimnagar, Andhra Pradesh-505884, IN
5 Anurag College of Pharmacy, Kodada, Nalgonda, Andhra Pradesh-501301, IN

Abstract

The entire wound healing process is a complex series of events that begins at the moment of injury and can continue for months to years. The stages of wound healing are inflammatory phase, proliferation phase, fibroblastic phase and maturation phase. The objective of our study is to investigate wound healing activity of the methanolic leaf extract of of Bauhinia purpurea Linn in rats.. The leaf extract of Bauhinia purpurea (200 mg/kg/day) was evaluated for its wound healing activity in albino rats using excision and incision wound models. Bauhinia purpurea leaf extract treated animals exhibit 83.42 % reduction in wound area when compared to controls which was 76.22 %. The extract treated wounds are found to epithelize faster as compared to controls. Significant (p<0.001) increase in granuloma breaking strength (485±34.64) was observed. The povidone iodine ointment was used as standard.

Keywords

Bauhinia purpurea, Wound Healing, Excision Wound, Incision Wound, Povidone Iodine.

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Sudhakara Reddy Pondugula ¹, Nataraj Narra ², J. Sunil ³, B. Srinivas ⁴

Affiliations
1 Dr. Reddys Laboratories, Bachupally, Hyderabad, A.P., IN
2 Mother Teresa College of Pharmaceutical Sciences, NFC Nagar, Ghatkesar, IN
3 Anurag Pharmacy College, Kodad, AP, IN
4 RS I Laboratories, Hyderabad, AP, IN

Abstract

Taking advantage of the specific hydrogen bonding interactions, stable and pH-responsive core-shell nanoparticles based on hydroxyethyl cellulose (HEC) and polymethacrylic acid (PMAA) networks, with a (D_h ) size ranging from 190 to 250 nm, can be efficiently prepared via facile one-step co-polymerization of methacrylic acid (MAA) and N,N'- methylenebisacrylamide (MBA) on HEC template in water. Using dynamic light scattering, electrophoretic light scattering, fluorescence spectrometry, thermo-gravimetric analysis, TEM, and AFM observations, the influence of crosslinker MBA as well as the reaction parameters were studied. The results show that after the introduction of crosslinker MBA, the nanoparticles became less compact; their size exhibited a smaller pH sensitivity, and their stability against pH value was improved greatly. Furthermore, the size, structure, and pH response of the nanoparticles can be adjusted via varying the reaction parameters: nanoparticles of smaller size, more compact structure, and higher swelling capacity were produced as pH value of the reaction medium increased or the HEC/MAA ratio decreased; while nanoparticles of smaller size, less compact structure and smaller swelling capacity were produced as the total feeding concentration increased.

Keywords

Core, Shell Nanoparticles, Synthesis, Template Polymerization, pH Sensitivity, Crosslinking.

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U. Samba Moorthy ¹, J. Sunil ², M. Sanjith Nath ²

Affiliations
1 Swami Ramananda Tirtha Institute of Pharmaceutical Sciences, Nalgonda, Andhra Pradesh, IN
2 Department of Pharmacy, Krishna University, Machilipatnam, Andhra Pradesh, IN

Abstract

A Simple and Precise HPLC method was developed for the estimation of Ibuprofen and Pseudoephedrine in pure and pharmaceutical dosage forms. The quantification was carried out using a Xterra RP₁₈ column 4.6 mm X 100 mm i.d, 3.5 μm particle size in isocratic mode, with mobile phase compressing of buffer and acetonitrile in the ratio of 70:30 (v/v) pH 7.2. The flow rate was 1.5 mL/min and the detection was carried out by UV detector at 210 nm. The retention times were 6.620 and 10.255 min for Pseudoephedrine and Ibuprofen, respectively. The method produced linear response at the concentration range of 62-180 ppm for Ibuprofen and 12-36 ppm Pseudoephedrine. The percentage recovery was found to be 99.73 and 100.63% for Ibuprofen and Pseudoephedrine, respectively. The method was validated by evaluation of different parameters.

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